Expression and functional characterization of recombinant soluble CD40 Ligand in prokaryotic systems
Our optimized prokaryotic system efficiently produces functional sCD40L without requiring eukaryotic post-translational modifications, providing a valuable tool for studying CD40L-mediated hemostasis and developing potential therapeutic applications.
Authors
- Wei Zhang (ORCID: https://orcid.org/0000-0001-6937-5562)
- Qi Xu
- Hanlin Liu (ORCID: https://orcid.org/0000-0002-2673-5289)
- Tao Hong (ORCID: https://orcid.org/0000-0002-2693-8991)
- Xue‐Zhong He (ORCID: https://orcid.org/0000-0003-1446-9996)
- Jianying Bai
- Suying Dang
Institutions
- Shanghai Jiao Tong University (CN)
- East China Normal University (CN)
Publication Details
- Journal
- Protein Expression and Purification
- Published
- 2025-12-19
- DOI
- https://doi.org/10.1016/j.pep.2025.106874
- Primary Topic
- Platelet Disorders and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00