Enhancement of Consolidated Bioprocessing for Bioethanol Conversion From Water Hyacinth Using the Wild and Mutant Bacterial Strains

Bacterial strains Hangateiclostridium thermocellum KSMK1203 and Lachnoclostridium phytofermentans KSM 1203 were subjected to physiochemical mutagenesis to improve their capacity for cellulose degradation, boost ethanol production, and enhance their resilience against various substrates and byproducts. This may lead to the development of strains that are stronger and more effective in producing biofuels. The study primarily concentrated on the use of NaClO 2 combined with an alkaline pretreatment for cellulose extraction and the fermentation of water hyacinth biomass through consolidated bioprocessing (CBP) utilizing both wild and mutant strains of H. thermocellum KSMK1203 and L. phytofermentans KSM 1203. Pretreatment significantly improves sugar extraction; mutant strains can further increase ethanol output even when working with untreated biomass. This fermentation method efficiently converts cellulose derived from water hyacinth biomass into bioethanol through a CBP. Both wild‐type and mutant strains promoted cellulase secretion to hydrolyze cellulose and produce bioethanol in a single reactor system. The results promote effectively utilizing water hyacinth to make bioethanol through CBP.