Multi‐Omics Analyses Reveal Divergent Molecular Mechanisms Underlying Plant Biomass Conversion by Five Fungi
Fungal plant biomass conversion (FPBC) is of great importance to the global carbon cycle and has been increasingly applied for the production of biofuel and biochemicals from lignocellulose. However, the comprehensive understanding of relevant molecular mechanisms in different fungi remains challenging. Here, we comparatively analyzed the transcriptome, proteome and metabolome profile of four ascomycetes and one basidiomycete fungi during their growth on two common agricultural feedstocks (soybean hulls and corn stover). We revealed strong time-, substrate- and species-specific responses at multi-omics levels for the tested fungi, highlighting species-specific carbon utilization approaches and evolutionary adaptation to environmental niches. Notably, a remarkable expressional diversity of lignocellulose degrading enzymes, sugar transporter and metabolic genes, as well as industrially relevant metabolites were identified across different fungi and cultivation conditions. The findings improves our understanding of complex molecular networks underlying FPBC and fungal ecological roles, offering novel insights that can guide future genetic engineering of fungi for valorization of agriculture waste into value-added bioproducts.
Authors
- Vivian Ng (ORCID: https://orcid.org/0000-0001-8941-6931)
- Sandra Garrigues (ORCID: https://orcid.org/0000-0002-4104-6246)
- Mao Peng (ORCID: https://orcid.org/0000-0003-1676-0242)
- Scott Baker (ORCID: https://orcid.org/0000-0001-5085-3106)
- Igor V. Grigoriev (ORCID: https://orcid.org/0000-0002-3136-8903)
- Tania Chroumpi
- Chaevien Clendinen (ORCID: https://orcid.org/0000-0002-1487-4126)
- Anna Lipzen (ORCID: https://orcid.org/0000-0003-2293-9329)
- Roland S. Kun (ORCID: https://orcid.org/0000-0002-4852-2081)
- María Victoria Aguilar Pontes (ORCID: https://orcid.org/0000-0002-3174-8834)
- Nikola Tolić (ORCID: https://orcid.org/0000-0003-3950-9130)
- Li Xu (ORCID: https://orcid.org/0000-0003-3782-8509)
- Jiali Meng
- Ronald P. de Vries (ORCID: https://orcid.org/0000-0002-4363-1123)
- Jiajia Li (ORCID: https://orcid.org/0000-0003-2894-6458)
Institutions
- Pacific Northwest National Laboratory (US)
- Lawrence Berkeley National Laboratory (US)
- Joint Genome Institute (US)
- Environmental Molecular Sciences Laboratory (US)
- Westerdijk Fungal Biodiversity Institute (NL)
- Joint BioEnergy Institute (US)
- University of California, Berkeley (US)
Publication Details
- Journal
- MicrobiologyOpen
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1002/mbo3.70201
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Energy
- Joint Genome Institute
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- China Scholarship Council
- Ministry of Infrastructure and Water Management
- Office of Science