Optimizing culture-free approaches to recover high quality <i>M. tuberculosis</i> genomic variation
Hybrid capture of diagnostic sputa samples efficiently generates accurate <i>Mtb</i> whole genome sequences and minority variant calls. Hybrid capture may offer an alternative to culture-based sequencing that could extend the coverage of genomic epidemiology studies.
Authors
- Allison F. Carey (ORCID: https://orcid.org/0000-0003-0937-4976)
- Paulo César Pereira dos Santos (ORCID: https://orcid.org/0000-0001-5470-6314)
- Salika M. Shakir (ORCID: https://orcid.org/0000-0003-4642-8574)
- Ted Cohen (ORCID: https://orcid.org/0000-0002-8091-7198)
- Jason Randolph Andrews (ORCID: https://orcid.org/0000-0002-5967-251X)
- Júlio Croda (ORCID: https://orcid.org/0000-0002-6665-6825)
- Katharine S. Walter (ORCID: https://orcid.org/0000-0003-0065-2204)
- Barun Mathema (ORCID: https://orcid.org/0000-0003-2772-479X)
- Caroline Colijn (ORCID: https://orcid.org/0000-0001-6097-6708)
Institutions
- Universidade Federal de Mato Grosso do Sul (BR)
- Simon Fraser University (CA)
- University of Utah (US)
- Yale University (US)
- ARUP Laboratories (United States) (US)
- Fundação Oswaldo Cruz (BR)
- Columbia University (US)
- Stanford University (US)
Publication Details
- Journal
- medRxiv
- Published
- 2025-12-18
- DOI
- https://doi.org/10.64898/2025.12.16.25342406
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00