High-throughput single-cell omics using semipermeable capsules
Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput single-cell omics methods and tools that efficiently probe the cellular phenotype and genotype. Here, we present a versatile technology based on semipermeable capsules (SPCs) tailored for a variety of high-throughput nucleic acid assays, including single-cell genome and mRNA sequencing, and fluorescence-activated cell sorting-based isolation of individual transcriptomes based on a nucleic acid marker of interest. Being biocompatible, the SPCs support single-cell cultivation and clonal expansion over long periods of time, thereby overcoming a fundamental limitation of droplet microfluidics platforms. Overall, the SPCs represent a customizable and broadly applicable tool for easy-to-use, scalable single-cell omics applications that are built on multistep biochemical reactions.
Authors
- Denis Baronas (ORCID: https://orcid.org/0000-0003-1878-0837)
- Greta Leonavičienė (ORCID: https://orcid.org/0000-0002-3008-9603)
- Justina Žvirblytė (ORCID: https://orcid.org/0000-0001-8175-504X)
- Karolis Šablauskas (ORCID: https://orcid.org/0000-0003-4166-0331)
- Simonas Juzėnas (ORCID: https://orcid.org/0000-0001-9293-0691)
- Linas Mažutis (ORCID: https://orcid.org/0000-0002-5552-6427)
- Karolis Goda (ORCID: https://orcid.org/0009-0000-8276-6883)
- Vytautas Kašėta (ORCID: https://orcid.org/0000-0001-9702-3062)
- Laimonas Griškevičius (ORCID: https://orcid.org/0000-0002-3731-1537)
- Simonas Norvaisis (ORCID: https://orcid.org/0009-0000-0154-7970)
- Vincenta Mikulenaite (ORCID: https://orcid.org/0009-0003-1009-1751)
Institutions
- Vilnius University (LT)
- State Research Institute Centre for Innovative Medicine (LT)
- National Cancer Institute (LT)
- Umeå University (SE)
Publication Details
- Journal
- Science
- Published
- 2025-12-18
- DOI
- https://doi.org/10.1126/science.ady7227
- Citations
- 3
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 2.42