Co-isolation of genetically distinct Burkholderia pseudomallei strains from a single patient in North Queensland
While investigating colony morphology variation within clinical isolates of Burkholderia pseudomallei (Bp) isolated from a single clinical infection, we identified two distinct strains (based on their multi-locus sequence type) from Bp TSV292. Reports of simultaneous co-infection are un-common, with only two studies in Thailand documenting such cases: (1) 2 out of 133 cases, and (2) 13 out of 59 patients infected with two to three polyclonal Bp strains exhibiting different genotypes. Here, we present the first comprehensive multi-omics characterization of co-infecting Bpstrains isolated from a single infection at a hospital in Townsville, Australia, in 2018. This finding impacts the design of future diagnosis and treatment of Bp.
Authors
- Piklu Roy Chowdhury (ORCID: https://orcid.org/0000-0003-4352-603X)
- Kay A. Ramsay (ORCID: https://orcid.org/0000-0002-6682-9351)
- Garry S. A. Myers (ORCID: https://orcid.org/0000-0002-4756-4810)
- Sarah Reed (ORCID: https://orcid.org/0000-0001-5219-9590)
- Miranda E. Pitt (ORCID: https://orcid.org/0000-0002-8255-4036)
- Pauline M. L. Coulon (ORCID: https://orcid.org/0000-0001-9482-2360)
- Aven Lee (ORCID: https://orcid.org/0000-0002-4171-8532)
- Patrick N. A. Harris (ORCID: https://orcid.org/0000-0002-2895-0345)
- Ian Gassiep (ORCID: https://orcid.org/0000-0003-3548-6963)
- Joyce To (ORCID: https://orcid.org/0000-0003-3482-4369)
- Edita Ritmejerytė (ORCID: https://orcid.org/0000-0002-8605-9198)
Institutions
- University of Technology Sydney (AU)
- The University of Queensland (AU)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-12-18
- DOI
- https://doi.org/10.1371/journal.pone.0338333
- Citations
- 1
- Primary Topic
- Burkholderia infections and melioidosis
- Type
- article
- Field-Weighted Citation Impact
- 1.35