Simulation of Nanoemulsion Stability in eLiposomes

eLiposomes are oil-in-water nanoemulsions encapsulated in the internal pool of liposomes. When creating such systems, the main challenge is understanding the conditions for the formation of various structures in the confined space of liposomes. In this study, we propose a method for calculating the attractive and repulsive forces between an oil droplet and the internal surface of a hollow sphere with the purpose to simulate the nanoemulsion stability in the interior of eLiposomes. The motion of oil droplets and their interaction with the internal surface of a liposome are simulated using Langevin dynamics. At a low liposome charge of –10 mV, oil droplets are adsorbed on the internal surface of liposomes to form structures that may be referred to as inverted colloidosomes. If the charge of the oil droplets in the nanoemulsion is also low and equal to –10 mV, the adsorbed oil droplets form regions with a dense hexagonal packing on the internal surface of the liposomes. When the charge on the oil droplets in the nanoemulsion is high and equal to –50 mV, the droplets repulse each other and are located at some distance, thereby forming a layer with a loose packing. Such multicompartment systems (inverted colloidosomes) are promising carriers for hydrophobic, hydrophilic, and amphiphilic drug compounds.