Impact of recombinant expression in Komagataella phaffii on the allergenic properties of the peanut allergen Ara h 2
An endogenous protease, like Kex2 from the expression system, can affect the structural integrity of the target protein, leading to a slightly altered epitope structure. Even this finding has little or no impact for diagnosis, a suitable expression system and a detailed physico- and immunochemical characterization of recombinant allergens prior to their use as a diagnostic tool are of great importance.
Authors
- Stephan Scheurer (ORCID: https://orcid.org/0000-0002-2859-562X)
- Andrea Wangorsch (ORCID: https://orcid.org/0000-0002-5184-5455)
- Melanie Albrecht (ORCID: https://orcid.org/0000-0002-5592-7853)
- Sonja Wolfheimer
- Jonas Lidholm (ORCID: https://orcid.org/0000-0002-7779-3411)
- Annette Jamin
- S. Vieths
Institutions
- Thermo Fisher Scientific (Sweden) (SE)
- Paul Ehrlich Institut (DE)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-12-18
- DOI
- https://doi.org/10.3389/fimmu.2025.1713823
- Primary Topic
- Food Allergy and Anaphylaxis Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00