Endogenous Biomarkers Analysis and False-Negative Results for SARSCov2 Using two Commercial RT-PCR Diagnostic Kits
Proper quality control measures are crucial for accurate SARS-CoV-2 detection. The study highlights the importance of selecting reliable diagnostic kits with high sensitivity and specificity to reduce false-negative results, particularly in cases with a low viral load or early stages of the disease. The internal RNA control can detect RNA degradation and help identify false-negative diagnoses, leading to better disease control and management. Further research is needed to improve the accuracy of COVID-19 diagnostic tests.
Authors
- Aida Gholoobi (ORCID: https://orcid.org/0000-0001-6399-1612)
- Aurora Heidar Alizadeh (ORCID: https://orcid.org/0000-0002-2455-2921)
- Toktam Dehghani (ORCID: https://orcid.org/0000-0003-3931-2880)
- Mahdieh Khoshakhlagh (ORCID: https://orcid.org/0000-0001-6676-4722)
- Samaneh Abolbashari (ORCID: https://orcid.org/0000-0002-4854-5822)
- Zahra Meshkat (ORCID: https://orcid.org/0000-0002-4032-7599)
- Mojtaba Meshkat (ORCID: https://orcid.org/0000-0003-0507-3295)
- Fatemeh Ghaemi
Institutions
- Mashhad University of Medical Sciences (IR)
- Islamic Azad University, Mashhad (IR)
Publication Details
- Journal
- Iranian journal of pathology
- Published
- 2025-12-18
- DOI
- https://doi.org/10.30699/ijp.2025.2053609.3417
- Primary Topic
- SARS-CoV-2 detection and testing
- Type
- article
- Field-Weighted Citation Impact
- 0.00