A new strategy of construction of porcine myocardial infarction model for preclinical research

Abstract The porcine model of myocardial infarction (MI) has been widely used to assess the feasibility, safety, and efficacy of prototype products in preclinical research. The heterogeneity of the left coronary artery (LCA) among pigs led to relatively low stability and repeatability, likely diminishing the accuracy of the preclinical research. Herein, we proposed a comprehensive strategy for establishing stable and reproducible porcine MI model based on LCA anatomical classification. Through precise analysis of coronary anatomical features via coronary angiography, we categorized the LCA into a nine‐type classification system and identified candidate subtypes for construction of porcine MI model. Utilizing a new embolic composite, we proposed subtype‐specific occlusion protocols to achieve stable vessel embolization within 3 s, effectively preventing blood flow from recanalization. This strategy significantly improved the efficiency, accuracy, and reproducibility in porcine MI induction, providing a more reliable experimental platform for preclinical research of prototype products.