A Cre-mediated copy number variant compromises the reliability of a <i>LoxP-STOP-LoxP</i> - <i>PLAG1</i> driven brain tumor model
Our work demonstrates the necessity of copy-number analysis when working with transgenic Cre-LoxP mouse models. Assessing CNVs should become a standard evaluation procedure when reporting new tumor models, preventing misleading conclusions that could dramatically impact the reliability of preclinical studies.
Authors
- Lena M. Kutscher (ORCID: https://orcid.org/0000-0002-1130-4582)
- Nina Hofmann (ORCID: https://orcid.org/0000-0001-8420-6281)
- T Fábián
- Sangita Pal (ORCID: https://orcid.org/0000-0002-4892-841X)
- Melanie Schoof (ORCID: https://orcid.org/0000-0002-1373-8130)
- Philipp Zimmer (ORCID: https://orcid.org/0000-0001-9631-4503)
- Jan Vaillant (ORCID: https://orcid.org/0009-0003-6283-9581)
- Jan Müller
- Michaela‐Kristina Keck (ORCID: https://orcid.org/0000-0002-3014-4676)
- Philipp Sievers (ORCID: https://orcid.org/0000-0003-3237-6021)
- Marc Zuckermann (ORCID: https://orcid.org/0000-0003-0148-3289)
- Ulrich Schüller (ORCID: https://orcid.org/0000-0002-8731-1121)
- Pratiti Bandopadhayay (ORCID: https://orcid.org/0000-0002-4175-4760)
- David Jones (ORCID: https://orcid.org/0000-0002-2036-5141)
- Andrea Wittmann
- Rameen Beroukhim
- Akosua Boakye-Yiadom
- Franziska Schelb
Institutions
- Broad Institute (US)
- Harvard University (US)
- Joint Graduate School of Energy and Environment (TH)
- Universität Hamburg (DE)
- German Cancer Research Center (DE)
- Heidelberg University (DE)
- University Hospital Heidelberg (DE)
- University Medical Center Hamburg-Eppendorf (DE)
- Kinderkrebs-Zentrum Hamburg (DE)
- National Center for Tumor Diseases (DE)
- Hopp Children's Cancer Center Heidelberg (DE)
- Dana-Farber Cancer Institute (US)
- Dana-Farber/Boston Children's Cancer and Blood Disorders Center (US)
- Heidelberg University (US)
Publication Details
- Journal
- Neuro-Oncology Advances
- Published
- 2025-12-05
- DOI
- https://doi.org/10.1093/noajnl/vdaf251
- Primary Topic
- Genomic variations and chromosomal abnormalities
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Brain Tumour Charity
- Deutsche Krebshilfe