Comparison of multispectral singlet oxygen luminescence dosimetry and singlet oxygen explicit dosimetry in artificial phantom

We demonstrate that singlet oxygen ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:msub> <mml:mrow> <mml:mmultiscripts><mml:mrow><mml:mi>O</mml:mi></mml:mrow> <mml:mprescripts></mml:mprescripts> <mml:none></mml:none> <mml:mrow><mml:mn>1</mml:mn></mml:mrow> </mml:mmultiscripts> </mml:mrow> <mml:mrow><mml:mn>2</mml:mn></mml:mrow> </mml:msub> </mml:mrow> </mml:math> ) detected through MSOLD can reliably quantify ROS concentrations in BPD-mediated PDT with accuracy comparable to the SOED model, which requires separate measurements of light fluence rate, photosensitizer concentration, and oxygen availability, followed by modeling to estimate the amount of reactive singlet oxygen. By contrast, MSOLD can also be a more cost-effective, simpler, and faster alternative to SOED as it directly measures the singlet oxygen luminescence to quantify reactive singlet oxygen. Under appropriate correction for tissue optical properties, MSOLD presents a promising, robust, and direct dosimetry solution for clinical PDT applications.