Computational and experimental analysis for the mismatched DNA recognition mechanism of TaqMutS
By sensing local conformational strains in DNA, Taq MutS utilizes a push-pull mechanism via the synergy of residues S57 and D472 to modulate π-stacking interactions between the key recognition residue F39 and DNA, thereby achieving specific mismatch-site recognition. Meanwhile, residues K61 and R473 fine-tune non-specific binding of Taq MutS through electrostatic synergy.
Authors
- Xiao Huan Liang (ORCID: https://orcid.org/0000-0002-9423-9201)
- Wenyang Jiang (ORCID: https://orcid.org/0000-0003-1255-5452)
- Haojie Sun (ORCID: https://orcid.org/0009-0004-0874-3543)
- Quanshun Li (ORCID: https://orcid.org/0000-0002-4115-7552)
- Yiyang Ding (ORCID: https://orcid.org/0000-0001-6031-1263)
- Yuxuan Li
Institutions
- Jilin University (CN)
Publication Details
- Journal
- Synthetic and Systems Biotechnology
- Published
- 2026-11-04
- DOI
- https://doi.org/10.1016/j.synbio.2026.10.011
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jilin Province Development and Reform Commission
- Royal Society
- Royal Society of Chemistry
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- People's Government of Jilin Province
- National Key Research and Development Program of China