A Rapid Assay for Hepatitis C Virus RNA Detection Using Reverse-Transcription Loop-Mediated Isothermal Amplification–Coupled CRISPR-Cas12b–Based Strategy
Our assay shows high specificity and sensitivity to detect HCV RNA directly from plasma within 45 min, and hence could be utilized for efficient screening and diagnosis of HCV infection globally.
Authors
- Karen L. Maxwell (ORCID: https://orcid.org/0000-0002-0211-5480)
- Jordan J. Feld (ORCID: https://orcid.org/0000-0003-2640-2211)
- Alan R. Davidson (ORCID: https://orcid.org/0000-0002-0744-9199)
- Avisha Chowdhury (ORCID: https://orcid.org/0000-0001-5301-2185)
- Muhammad Atif Zahoor (ORCID: https://orcid.org/0000-0003-2302-3689)
- Haley D.M. Wyatt (ORCID: https://orcid.org/0000-0001-9995-1057)
- Adam J. Gehring (ORCID: https://orcid.org/0000-0003-1150-5840)
- Bianca Garcı́a (ORCID: https://orcid.org/0000-0002-1081-5452)
- Nahla Fadl ElMawla (ORCID: https://orcid.org/0009-0000-6201-1512)
- Ayoub Mahassine (ORCID: https://orcid.org/0009-0006-0345-5053)
Institutions
- University Health Network (CA)
- University of Toronto (CA)
- Toronto General Hospital (CA)
Publication Details
- Journal
- The Journal of Infectious Diseases
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1093/infdis/jiaf609
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00