Diversity of Sordariales Fungi: Identification of Seven New Species of Naviculisporaceae Through Morphological Analyses and Genome Sequencing
Thanks to next-generation sequencing (NGS) technologies, the diversity of fungi can now be investigated through the analysis of their genome sequences. <i>Naviculisporaceae</i> is a family within the <i>Sordariales</i>, whose diversity is not well-known, with only one genome sequence published for this family. Here, we report on the isolation and cultivation of 20 new strains of <i>Naviculisporaceae</i>. Their genome sequences, as well as those of the five commercially available strains, were determined, thus providing complete genome sequences for 25 new <i>Naviculisporaceae</i> strains. Species delimitation was conducted using a combination of (1) ITS + LSU phylogenetic analysis of the new isolates along with other known species of the family, (2) comparisons between DNA barcode sequences of the new strains with those of the known species, and (3) average genome-wide nucleotide identity calculation. We built a phylogenomic tree and studied the organization of the mating-type locus. In vitro fruiting was obtained for 16 strains, enabling the definition of seven new species, namely <i>Pseudorhypophila gallica</i>, <i>Pseudorhypophila guyanensis Rhypophila alpibus</i>, <i>Rhypophila brasiliensis</i>, <i>Rhypophila camarguensis</i>, <i>Rhypophila reunionensis</i> and <i>Rhypophila thailandica</i>, as well as two new combinations, namely <i>Pseudorhypophila latipes</i> and <i>Pseudorhypophila oryzae</i>. Eight strains for which in vitro fruiting was not obtained may belong to additional new species. These results expand the known diversity of the <i>Naviculisporaceae</i> and greatly enlarge the genomic data available for the family.
Authors
- Pierre Gladieux (ORCID: https://orcid.org/0000-0003-1929-1576)
- Vivian Ng (ORCID: https://orcid.org/0000-0001-8941-6931)
- S. L. Calhoun
- Anna Lipzen (ORCID: https://orcid.org/0000-0003-2293-9329)
- Philippe Silar (ORCID: https://orcid.org/0000-0003-0104-987X)
- Sandrine Cros‐Arteil (ORCID: https://orcid.org/0000-0002-4079-1551)
- Richard D. Hayes (ORCID: https://orcid.org/0000-0002-5236-7918)
- Robert Riley (ORCID: https://orcid.org/0000-0003-0224-0975)
- Tatiana Giraud (ORCID: https://orcid.org/0000-0002-2685-6478)
- Christophe Lalanne (ORCID: https://orcid.org/0000-0003-1915-2824)
- Jasmyn Pangilinan (ORCID: https://orcid.org/0000-0001-7966-3496)
- Lucas Bonometti (ORCID: https://orcid.org/0009-0000-6584-216X)
- Igor V. Grigoriev (ORCID: https://orcid.org/0000-0002-3136-8903)
- Valérie Gautier
- Narumon Tangthirasunun
Institutions
- Centre National de la Recherche Scientifique (FR)
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Los Alamos National Laboratory (US)
- Lawrence Berkeley National Laboratory (US)
- Joint Genome Institute (US)
- Université Paris Cité (FR)
- AgroParisTech (FR)
- Université Paris-Saclay (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Ecologie, Société, Evolution (FR)
- Laboratoire Interdisciplinaire des Énergies de Demain (FR)
- Génétique Quantitative et Évolution Le Moulon (FR)
- Ecologie & Evolution (FR)
- Institut Agro Montpelier (FR)
- Services déconcentrés d'appui à la recherche Occitanie-Montpellier (FR)
- Institut de Recherche pour le Développement (FR)
- Plant Health Institute de Montpellier (FR)
- King Mongkut's Institute of Technology Ladkrabang (TH)
- University of California, Berkeley (US)
Publication Details
- Journal
- Journal of Fungi
- Published
- 2025-12-12
- DOI
- https://doi.org/10.3390/jof11120880
- Primary Topic
- Plant Pathogens and Fungal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00