An optimized protocol for plant extracellular vesicles isolation from Ophiopogon japonicus root: a comparative evaluation based on miRNA cargo
This study establishes a robust and adaptable pipeline for isolating high-quality, functionally intact plant EVs from challenging plant root tissues. The optimized protocol effectively addresses the critical methodological challenges of yield and purity, enabling reliable downstream functional characterization and advancing therapeutic investigations of plant-derived EVs.
Authors
- Xin Zhao (ORCID: https://orcid.org/0000-0001-9798-7865)
- Tao Zhou (ORCID: https://orcid.org/0000-0002-8869-7599)
- Xiao Yang (ORCID: https://orcid.org/0000-0002-9573-8782)
- Qi Zheng (ORCID: https://orcid.org/0000-0002-0138-0506)
- Siyu Chen (ORCID: https://orcid.org/0000-0003-1156-7910)
- Feng Li (ORCID: https://orcid.org/0000-0003-3636-2962)
- Binjie Xu (ORCID: https://orcid.org/0000-0003-1349-7258)
- Zihan Li (ORCID: https://orcid.org/0009-0004-3839-0611)
- Yuntong Ma (ORCID: https://orcid.org/0000-0001-6554-5317)
- Fengqi Lv
Institutions
- Chengdu University of Traditional Chinese Medicine (CN)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-12-14
- DOI
- https://doi.org/10.1186/s13007-025-01481-7
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81