Addressing nonspecific interactions in a Gyrolab anti-drug antibody (ADA) assay: a case study on method optimization to improve repeatability
Careful optimization of assay conditions, particularly buffer composition and pH successfully resolved issues related to the nonspecific interactions and enhanced assay robustness. Optimized Gyrolab-based immunoassay provided a reliable method for ADA detection, supporting immunogenicity assessment in a clinical development program.
Authors
- J.S. Chappell (ORCID: https://orcid.org/0009-0002-1685-5241)
- Dorota Jaros
- Agata Sakowicz (ORCID: https://orcid.org/0000-0002-5083-2046)
- Małgorzata Urbaniak
- Eliza Kęsy-Siwik (ORCID: https://orcid.org/0000-0001-5126-7336)
Institutions
- Protein Technologies (United Kingdom) (GB)
- Medical University of Lodz (PL)
- Mabion (Poland) (PL)
Publication Details
- Journal
- Bioanalysis
- Published
- 2025-12-02
- DOI
- https://doi.org/10.1080/17576180.2025.2601860
- Primary Topic
- Biosimilars and Bioanalytical Methods
- Type
- article
- Field-Weighted Citation Impact
- 0.00