Middle-down proteomics: the pursuit for longer peptides

MDP offers significant potential to uncover the 'dark' proteome, including PTM-rich regions and proteoforms undetectable by traditional workflows. However, a focused effort on improving high-throughput workflows will require optimizations to enzyme selection, LC-MS parameters, peptide ionization, ion mobility, ion fragmentation, and tailored algorithms are essential to drive MDP's adoption. Only then will deeper proteomic insights and breakthroughs in biological research be obtained.