Immunochromatographic Test System for the Determination of Bisphenol A with Magnetic Concentration

Bisphenol A (BPA) is widely used as a hardener in the plastics industry. However, its release and circulation in ecosystems lead to contamination of drinking water, which negatively impacts the human endocrine system. Therefore, there is a need for simple and efficient tools for monitoring BPA. This study presents a combination of two approaches for this purpose: the use of magnetic particles (MP) as antibody carriers and immunochromatographic test strips for detecting labeled immune complexes. The visual limit of detection (LOD) of BPA was 10 μg/mL, the instrumental LOD was 55 ng/mL, and the working range of detectable concentrations was 0.1–10 μg/mL. The efficacy of using the MP conjugate for analyte concentration was demonstrated, resulting in an approximately 200-fold reduction of the LOD. The developed immunochromatographic assay (ICA) is suitable for the qualitative monitoring and quantitative determination of BPA in drinking and natural water samples (with 94–106% recoveries).