An insight into the aerobic bacteria and their antibiogram isolated from pus samples

Introduction: Pyogenic wound infections are generally due to local inflammation in the tissues mainly caused by pyogenic bacteria and the pathogens isolated from these infections and their antibiotic susceptibility pattern varies in different geographical areas where inappropriate local knowledge regarding the bacteriological profile and susceptibility pattern will not only lead to prolonged hospital stay, these patients can transfer these drug resistant bacteria to other patients and also to health care providers. Aim: To isolate the aerobic bacterial pathogens from pus samples and study their antibiotic susceptibility pattern. Materials and Methods: A total of 370 pus samples collected by sterile aspiration or sterile swab were processed in the Department of Microbiology by microscopy and culture by standard protocol and the bacterial pathogens were identified and antibiotic susceptibility testing of all isolates was done by Kirby bauer disc diffusion method using Mueller-Hinton agar and interpreted according to CLSI guidelines 2021. Results: Out of 370 pus samples tested 264 (71.35%) were culture positive and 106 (28.65%) were culture negative even after 48 hours of aerobic incubation at 370C. Among the culture positive 161 (60.98%) were Gram negative bacilli in which the predominant isolate was Escherichia coli (38.5%) followed by Pseudomonas aeruginosa (28.84%) and 103 (39.02%) were Gram positive cocci in which the predominant isolate was Staphylococcus aureus (MSSA) 33.98% followed by Staphylococcus aureus (MRSA) 31%. Conclusion: Pyogenic wound infections are very common and are also an important cause of morbidity. The present study will guide the clinicians to know the prevalence of bacterial pathogens and their antibiotic sensitivity pattern encountered in the pus samples as antimicrobial resistance is a challenge for the clinicians to treat these infections.