Evaluation of T-NGS, Xpert Ultra, and Line Probe Assay on Clinical Samples for the Diagnosis of Drug-Resistant Pulmonary Tuberculosis in Rio de Janeiro
Xpert Ultra and LPA-1 are effective for the rapid detection of rifampicin resistance but have limitations for isoniazid and second-line drugs. T-NGS improved the detection of low-level resistance, heteroresistance, and mixed infections, supporting its implementation in reference laboratories for comprehensive DR-TB diagnosis.
Authors
- Pedro Eduardo Almeida da Silva (ORCID: https://orcid.org/0000-0003-1666-1295)
- Martha Maria Oliveira (ORCID: https://orcid.org/0000-0002-0064-387X)
- Luís Cristóvão Pôrto (ORCID: https://orcid.org/0000-0003-1499-1821)
- E. Ribeiro (ORCID: https://orcid.org/0009-0003-4835-1571)
- Afrânio Lineu Kritski (ORCID: https://orcid.org/0000-0002-5900-6007)
- Danielle Secco (ORCID: https://orcid.org/0000-0002-9514-1871)
- Marcela Bhering (ORCID: https://orcid.org/0000-0002-1568-026X)
- Elizabete Oliveira Ribeiro (ORCID: https://orcid.org/0000-0001-9572-7592)
- Anna Karla Silveira (ORCID: https://orcid.org/0009-0003-3749-3144)
- Miguel Viveiros (ORCID: https://orcid.org/0000-0001-9676-6251)
- Tathiany Igreja-Silva (ORCID: https://orcid.org/0000-0002-6140-7360)
- Carolyne Lalucha (ORCID: https://orcid.org/0000-0001-5022-9012)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Universidade Federal do Rio Grande (BR)
- Universidade do Estado do Rio de Janeiro (BR)
- Fundação Oswaldo Cruz (BR)
- Universidade Nova de Lisboa (PT)
Publication Details
- Journal
- Revista da Sociedade Brasileira de Medicina Tropical
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1590/0037-8682-0358-2025
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00