A novel PCR-free hybridization method for titering gene therapy vectors in crude cell lysates and various in-process matrices

Maintaining the accuracy and efficiency of AAV genome titer quantification is crucial in gene therapy manufacturing. This 14-minute video presentation, accompanied by a poster summary, explores a novel PCR-free assay that enhances speed, reliability, and flexibility. By utilizing DNA hybridization, immunochemistry, and bio-layer interferometry (BLI), this assay simplifies titer quantification and improves workflow efficiency. NEW INTERACTIVE FORMAT Listen to our expert speaker. Watch the video. Read the summary. Focus in on the data. Download the PDF. It has never been easier to quickly access the information you want. All in one place—at the click of a button.

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Publication Details

Journal
Cell and Gene Therapy Insights
Published
2025-04-04
DOI
https://doi.org/10.18609/cgti.2025.041
Primary Topic
Virus-based gene therapy research
Type
article
Field-Weighted Citation Impact
0.00
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article

A novel PCR-free hybridization method for titering gene therapy vectors in crude cell lysates and various in-process matrices

Wai Choi
Cell and Gene Therapy Insights
Virus-based gene therapy research
article

A novel PCR-free hybridization method for titering gene therapy vectors in crude cell lysates and various in-process matrices

Wai Choi
article en

Abstract

Maintaining the accuracy and efficiency of AAV genome titer quantification is crucial in gene therapy manufacturing. This 14-minute video presentation, accompanied by a poster summary, explores a novel PCR-free assay that enhances speed, reliability, and flexibility. By utilizing DNA hybridization, immunochemistry, and bio-layer interferometry (BLI), this assay simplifies titer quantification and improves workflow efficiency. NEW INTERACTIVE FORMAT Listen to our expert speaker. Watch the video. Read the summary. Focus in on the data. Download the PDF. It has never been easier to quickly access the information you want. All in one place—at the click of a button.

Cell and Gene Therapy InsightsVol. 11(3)
Bio-Medical Science (South Korea) (KR)
Openalex Percentile: Top 58%
Virus-based gene therapy research
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