Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis
Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including acute myeloid leukemia (AML), which exhibits aberrant lipid metabolism and chemoresistance. Here, we describe an effective protocol for the co-isolation of LDs and mitochondria from lipid-deficient cell types, e.g., AML cells. We detail procedures for the isolation, quality verification, and results from mass spectrometry-based lipidomic analysis. This protocol enables the functional study of organellar lipidomic regulation in AML.
Authors
- Hiroki Akiyama (ORCID: https://orcid.org/0000-0003-2627-1055)
- G. Lavender Hackman (ORCID: https://orcid.org/0000-0001-7439-5138)
- Jo Ishizawa (ORCID: https://orcid.org/0000-0001-8566-7370)
- Kazuharu Kamachi (ORCID: https://orcid.org/0000-0002-7346-4864)
- Michael Andreeff (ORCID: https://orcid.org/0000-0002-1144-1958)
- Alessia Lodi (ORCID: https://orcid.org/0000-0002-2104-5713)
- Liang Zhang (ORCID: https://orcid.org/0000-0001-9218-828X)
- Ran Zhao (ORCID: https://orcid.org/0000-0002-5887-5980)
- Shuxin Dong
- John D. Nguyen (ORCID: https://orcid.org/0000-0002-3848-590X)
- Saurabh Kumar Gupta
Institutions
- The University of Texas MD Anderson Cancer Center (US)
- The University of Texas at Austin (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2025.104257
- Citations
- 1
- Primary Topic
- Lipid metabolism and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 1.11