Influence of growth factors and different supplements on metabolic response and productivity of IgA-producing CHO cell lines

IgA antibodies have great potential for use in novel cancer immunotherapy based on recombinant monoclonal antibodies. This antitumor application is related to the effector functions of IgA. IgA subisotypes have special effector function features explained by a more complex glycosylation process compared to IgG antibodies. Studying of interrelations of metabolites, glucose consumptions, cell growth and antibody production can allow to get better control of antibody glycosylation and product quality. Reducing of metabolites in culture have influence on productivity and probably on glycosylation and can be achieved through process optimization or metabolic engineering. IgA1 and IgA2m1 have different effector functions associated with distinct glycosylation profiles. The aim of our study was to evaluate the effect of human growth factors such as hNGF, hEGF, and FGF-b combined with some supplements on metabolic level and production of recombinant IgA antibodies. The results demonstrated that combination growth factors and succinic acid improves metabolism of CHO cells producing recombinant monoclonal antibody IgA isotype, especially for IgA2m1producing cell line. These results suggest that combining hNGF, hEGF, and succinic acid supplementation has the potential to be used as effective additives to cell media for increasing the cell culture density, and optimization of IgA1-IgA2 cell line productivity.