Standardizing microbiome research: interlaboratory validation of SOPs for sample preparation and DNA extraction from food and environmental ecosystems
This study underlines the importance of standardized protocols in microbiome research. The validated Standard Operating Procedures developed by the SUS-MIRRI.IT project demonstrate robustness and reproducibility across diverse ecosystems, providing a foundation for future microbiome studies. The adoption of these protocols will enhance data comparability and support large-scale meta-analyses in food systems microbiome research.
Authors
- Silvia Lampis (ORCID: https://orcid.org/0000-0002-1686-3076)
- Ciro Sannino (ORCID: https://orcid.org/0000-0002-8611-9306)
- Massimo Ferrara (ORCID: https://orcid.org/0000-0002-4751-8478)
- Mattia Pia Arena (ORCID: https://orcid.org/0000-0002-2027-7344)
- Luca Cocolin (ORCID: https://orcid.org/0000-0003-4799-7074)
- Giuseppe Gallo (ORCID: https://orcid.org/0000-0003-4727-5653)
- Benedetta Turchetti (ORCID: https://orcid.org/0000-0002-7370-3028)
- Raimondo Gaglio (ORCID: https://orcid.org/0000-0002-2080-0836)
- Annamaria Bevivino (ORCID: https://orcid.org/0000-0003-4277-7048)
- Rosa Alduina (ORCID: https://orcid.org/0000-0003-1054-6915)
- Tiziana Di Renzo (ORCID: https://orcid.org/0000-0002-6036-3044)
- Marco Andreolli (ORCID: https://orcid.org/0000-0002-0972-6428)
- Luciana De Vero (ORCID: https://orcid.org/0000-0002-9887-9994)
- Maria Gullo (ORCID: https://orcid.org/0000-0002-9908-8254)
- Francesco Aloi (ORCID: https://orcid.org/0000-0002-0876-0763)
- Roberta Coronas
- D. Spadaro (ORCID: https://orcid.org/0000-0001-5207-9345)
- Valeria Tatangelo (ORCID: https://orcid.org/0009-0002-1983-5350)
- Marco Garello (ORCID: https://orcid.org/0000-0002-1607-4149)
- Teresa Zotta (ORCID: https://orcid.org/0000-0001-8228-6804)
- Paola Quatrini (ORCID: https://orcid.org/0000-0001-7638-2648)
- Vittorio Capozzi (ORCID: https://orcid.org/0000-0002-0717-0753)
- Giacomo Zara (ORCID: https://orcid.org/0000-0001-5302-7424)
- Andrea Visca (ORCID: https://orcid.org/0000-0002-1795-3532)
- Marilisa Giavalisco (ORCID: https://orcid.org/0000-0003-0525-7799)
- Anna Reale (ORCID: https://orcid.org/0000-0001-5576-257X)
- Rosa Guarcello (ORCID: https://orcid.org/0000-0003-0162-8353)
- Gianmarco Mugnai (ORCID: https://orcid.org/0000-0002-0130-7683)
- Ilario Ferrocino (ORCID: https://orcid.org/0000-0002-1657-0054)
- Andrea Franzetti (ORCID: https://orcid.org/0000-0003-1279-9940)
- Fiorella Sbarra
- Giovanna Cristina Varese
- E Bruno (ORCID: https://orcid.org/0009-0007-3359-9282)
Institutions
- University of Verona (IT)
- University of Modena and Reggio Emilia (IT)
- University of Padua (IT)
- University of Sassari (IT)
- University of Basilicata (IT)
- University of Perugia (IT)
- Institute of Food Science (IT)
- Institute of Sciences of Food Production (IT)
- National Agency for New Technologies Energy and Sustainable Economic Development (GB)
- National Research Council (IT)
- University of Turin (IT)
- University of Milano-Bicocca (IT)
- University of Palermo (IT)
Publication Details
- Journal
- Environmental Microbiome
- Published
- 2025-12-12
- DOI
- https://doi.org/10.1186/s40793-025-00833-z
- Citations
- 3
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 5.15