Evaluation of reference genes for accurate qRT-PCR normalization in bitter gourd across different tissues and stress conditions
By broadening the evaluation framework, this study systematically validated the universality and reliability of reference genes under diverse scenarios. These findings provide a robust set of internal controls for accurate qRT-PCR analyses in bitter gourd and will improve the reproducibility of future molecular studies in this species.
Authors
- Zihua Zhou (ORCID: https://orcid.org/0000-0002-8337-5443)
- Libo Tian (ORCID: https://orcid.org/0009-0008-9519-099X)
- Xuzhen Li (ORCID: https://orcid.org/0000-0003-3670-6657)
- Shihan Zeng (ORCID: https://orcid.org/0000-0003-3867-1849)
- Yang Dong (ORCID: https://orcid.org/0000-0001-6212-3055)
- Xiao‐Ou Shu (ORCID: https://orcid.org/0000-0002-0711-8314)
- Huilin Ma (ORCID: https://orcid.org/0000-0003-4238-9475)
- Feifan Chen (ORCID: https://orcid.org/0000-0003-3634-9493)
- Tian Xie (ORCID: https://orcid.org/0000-0003-2442-7941)
- Sang Shang
Institutions
- Hainan University (CN)
Publication Details
- Journal
- BMC Plant Biology
- Published
- 2025-12-12
- DOI
- https://doi.org/10.1186/s12870-025-07612-5
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China