Application of spectroscopic methods for direct characterization of photosynthetic pigments and inert intracellular components in the model purple non sulfur bacterium Rhodospirillum rubrum
This innovative combination of spectroscopic techniques reduces sample manipulation, preserves cellular integrity and provides rapid, precise, and environmentally friendly analysis of microbial metabolites in their natural physiological conditions. The demonstrated workflow is broadly applicable to biological samples where maintaining biomolecular integrity is crucial, and it has strong potential for applications in process analytical technology and industrial biotechnology.
Authors
- Stanislav Obruča (ORCID: https://orcid.org/0000-0002-9270-195X)
- Ota Samek (ORCID: https://orcid.org/0000-0001-8188-7608)
- Manfred Zinn (ORCID: https://orcid.org/0000-0003-3565-3872)
- Petr Sedláček (ORCID: https://orcid.org/0000-0002-6211-9643)
- Eva Slaninová
- Markéta Benešová
- Hugo Fleuriot-Blitman
- Martin Koller (ORCID: https://orcid.org/0000-0002-9251-1822)
- Viktorie-Alexandra Pacasova
Institutions
- University of Graz (AT)
- Nawi Graz (AT)
- Czech Academy of Sciences, Institute of Scientific Instruments (CZ)
- HES-SO Valais-Wallis (CH)
- Brno University of Technology (CZ)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-12-14
- DOI
- https://doi.org/10.1186/s12934-025-02876-w
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00