Comparative analysis of liquid and gel platelet rich plasma from apheresis and buffy coat in wound healing

Platelet-Rich Plasma (PRP) is widely used in wound care and tissue regeneration, yet variability in its preparation and delivery has hindered clinical standardization. This study compares the regenerative potential of PRP formulations derived from apheresis (Aphe PRP) and buffy-coat (BC PRP), in both liquid and gel forms. We characterized the seven-day release profile of platelet-derived effectors and assessed the impact of PRP on key wound-healing cellular processes-proliferation, migration, and differentiation-using keratinocytes, fibroblasts, and microvascular endothelial cell models. Our findings revealed a sustained effector release over seven days, with liquid PRPs showing significantly higher release levels than gels, regardless of PRP source. Aphe and BC PRPs exhibited comparable effects on cell proliferation, migration, and differentiation. However, gel PRPs were less effective than liquid PRPs in promoting proliferation and migration, although both forms significantly enhanced regeneration compared to untreated controls. Notably, differentiation effects were similar across all PRP types. These results suggest Aphe and BC PRPs are interchangeable for wound care, with liquid PRPs offering superior regenerative effects in early treatment stages. This study highlights the need for standardized PRP preparation and application protocols to optimize therapeutic outcomes.