Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs
Future drive designs requiring a larger number of gRNAs could potentially be improved. The design relying on extended homology arms may not represent an optimal strategy. However, population-level multiplexing gRNAs could serve as a promising alternative, enhancing efficiency while maintaining tolerance to functional resistance.
Authors
- Peixin Wu (ORCID: https://orcid.org/0000-0002-1288-0610)
- Weizhe Chen (ORCID: https://orcid.org/0009-0000-6239-4613)
- Jackson Champer (ORCID: https://orcid.org/0000-0002-3814-3774)
Institutions
- Center for Life Sciences (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- BMC Biology
- Published
- 2025-12-12
- DOI
- https://doi.org/10.1186/s12915-025-02488-1
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81