Complete genome sequence and genetic features of a novel Pseudomonas sp. isolate (CAM1A) from tsetse fly gut captured in Dodeo, Cameroon

Bacteria of the genus Pseudomonas are Gram-negative and comprise of at least 107 diverse species. Certain members of the genus Pseudomonas emerged as major opportunistic human pathogens, and others have been used as biocontrol agents. Tsetse flies, the biological vectors of African trypanosomes, harbour diverse communities of microorganisms. Tsetse microbiota is of interest because of their possible effect on vector competence. The aim of this study was to explore genetic features of a novel Pseudomonas species strain CAM1A isolated from tsetse caught in Dodeo, Cameroon. The bacterium was isolated from tsetse fly, and the whole genome was sequenced on the Illumina Miseq platform. Sequences were assembled with SPAdes and annotated using RAST and PATRIC annotation pipelines. The draft genome, made of 61 contigs was found to be at least 5,848,000 bp long, with 5,339 coding sequences, a DNA G + C content of 64.3%, and at least 80 tRNA + rRNA genes. No plasmid was found. Taxonomic analysis based on whole-genome sequences (ANI and GGDC) and MLSA indicated that CAM1A represents a putative novel species closely related to Pseudomonas soli. Several antimicrobial resistance and efflux-mediated resistance genes were predicted, suggesting its ability to evade many antimicrobial therapies. Determination of secondary metabolites within the genome revealed seven different secondary metabolites distributed on 11 biosynthetic gene clusters. Various virulence factors were found, but the isolate was predicted to be a non-human pathogen.