Rapid Visual Tracking of Glyphosate Contamination by a Cu ²⁺ ‐Mediated Nitrobenzodiazole Fluorescent Sensor

Agrochemicals such as glyphosate are widely applied to enhance crop yields, yet their excessive use has caused severe environmental pollution and raised public health concerns. Effective monitoring of glyphosate residues is thus crucial for safeguarding food safety and environmental quality. Here, it reports the design and synthesis of a nitrobenzodiazole (NBD)‐based fluorophore (NBPA), incorporating a bis(pyridin‐2‐ylmethyl)amine (BPA) moiety. This moiety serves a dual function: It provides a binding site for Cu 2+ while simultaneously modulating the intramolecular charge transfer (ICT) process. NBPA selectively coordinates with Cu 2+ , resulting in fluorescence quenching, while glyphosate efficiently displaces Cu 2+ from the NBPA–Cu 2+ complex through competitive coordination. This restores the ICT process and produces a pronounced fluorescence “turn‐on” signal, enabling rapid and visible detection of glyphosate. The probe shows high sensitivity, excellent selectivity, and a fast response within 40 s and can be incorporated into test strips for convenient on‐site monitoring of glyphosate residues in food and environmental samples (fluorescent color changes from blue–purple to yellow–green under a 365 nm ultraviolet lamp). This work provides a simple, cost‐effective, and highly responsive fluorescent sensing strategy for glyphosate, offering a promising tool for environmental monitoring and food safety assessment.