Dual-mode microscopic imaging enabled by a single liquid crystal lens

Dual-mode imaging technology that simultaneously acquires bright-field and phase-contrast images is attracting increasing attention, as it provides a more comprehensive and dynamic approach to studying biological processes. However, conventional implementations face two major limitations. On the one hand, bright-field and spiral phase contrast (SPC) imaging typically relies on different optical hardware, requiring mechanical switching between imaging modes. On the other hand, traditional optical components generally serve a single function, resulting in bulky systems that are difficult to miniaturize and integrate with existing platforms. This work presents an integrated electrically controlled Pancharatnam-Berry phase liquid crystal (LC) lens capable of enabling multi-wavelength dual-mode dual-channel parallel ( MD 2 ) imaging. The MD 2 LC lens combines vortex phase modulation, focusing, and beam splitting functions within a single device, enabling simultaneous bright-field and SPC imaging over a broad spectral range without any mechanical switching. Experimental validation on biological cell samples demonstrates that the MD 2 LC lens can extract both transmittance information and high-contrast edge details in parallel. The proposed scheme provides a technological pathway for multi-dimensional, non-invasive biological imaging and holds potential for applications in biomedical diagnostics, life-science research, and the development of portable imaging systems.

Authors

Publication Details

Journal
Photonics Research
Published
2025-12-12
DOI
https://doi.org/10.1364/prj.580996
Primary Topic
Digital Holography and Microscopy
Type
article
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Dual-mode microscopic imaging enabled by a single liquid crystal lens

Xiangsheng Xie, Haoxu Guo, Yaqin Zhou, Xinyi Zhou et al.
Photonics Research
Digital Holography and Microscopy
article

Dual-mode microscopic imaging enabled by a single liquid crystal lens

Xiangsheng Xie, Haoxu Guo, Yaqin Zhou, Xinyi Zhou, Mingwei Zhuang, Jingjing Wang, Xiaoxue Zhang
article en

Abstract

Dual-mode imaging technology that simultaneously acquires bright-field and phase-contrast images is attracting increasing attention, as it provides a more comprehensive and dynamic approach to studying biological processes. However, conventional implementations face two major limitations. On the one hand, bright-field and spiral phase contrast (SPC) imaging typically relies on different optical hardware, requiring mechanical switching between imaging modes. On the other hand, traditional optical components generally serve a single function, resulting in bulky systems that are difficult to miniaturize and integrate with existing platforms. This work presents an integrated electrically controlled Pancharatnam-Berry phase liquid crystal (LC) lens capable of enabling multi-wavelength dual-mode dual-channel parallel ( MD 2 ) imaging. The MD 2 LC lens combines vortex phase modulation, focusing, and beam splitting functions within a single device, enabling simultaneous bright-field and SPC imaging over a broad spectral range without any mechanical switching. Experimental validation on biological cell samples demonstrates that the MD 2 LC lens can extract both transmittance information and high-contrast edge details in parallel. The proposed scheme provides a technological pathway for multi-dimensional, non-invasive biological imaging and holds potential for applications in biomedical diagnostics, life-science research, and the development of portable imaging systems.

Photonics ResearchVol. 14(4)
Openalex Percentile: Top 67%
Digital Holography and Microscopy
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