Dual-mode microscopic imaging enabled by a single liquid crystal lens
Dual-mode imaging technology that simultaneously acquires bright-field and phase-contrast images is attracting increasing attention, as it provides a more comprehensive and dynamic approach to studying biological processes. However, conventional implementations face two major limitations. On the one hand, bright-field and spiral phase contrast (SPC) imaging typically relies on different optical hardware, requiring mechanical switching between imaging modes. On the other hand, traditional optical components generally serve a single function, resulting in bulky systems that are difficult to miniaturize and integrate with existing platforms. This work presents an integrated electrically controlled Pancharatnam-Berry phase liquid crystal (LC) lens capable of enabling multi-wavelength dual-mode dual-channel parallel ( MD 2 ) imaging. The MD 2 LC lens combines vortex phase modulation, focusing, and beam splitting functions within a single device, enabling simultaneous bright-field and SPC imaging over a broad spectral range without any mechanical switching. Experimental validation on biological cell samples demonstrates that the MD 2 LC lens can extract both transmittance information and high-contrast edge details in parallel. The proposed scheme provides a technological pathway for multi-dimensional, non-invasive biological imaging and holds potential for applications in biomedical diagnostics, life-science research, and the development of portable imaging systems.
Authors
- Xiangsheng Xie (ORCID: https://orcid.org/0000-0003-3337-6955)
- Haoxu Guo (ORCID: https://orcid.org/0000-0002-6679-3790)
- Yaqin Zhou (ORCID: https://orcid.org/0000-0001-5664-0603)
- Xinyi Zhou (ORCID: https://orcid.org/0000-0003-1126-6333)
- Mingwei Zhuang (ORCID: https://orcid.org/0000-0002-5771-6603)
- Jingjing Wang (ORCID: https://orcid.org/0000-0002-4786-9227)
- Xiaoxue Zhang (ORCID: https://orcid.org/0009-0000-8492-4187)
Publication Details
- Journal
- Photonics Research
- Published
- 2025-12-12
- DOI
- https://doi.org/10.1364/prj.580996
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 0.00