DETERMINATION OF DOXORUBICIN BASED ON QUENCHING OF LUMINESCENCE OF ALLOYED QUANTUM DOTS

A sensitive, simple method for determining doxorubicin (Dox) in biological fluids, which does not require complex sample preparation, has been developed based on the quenching of luminescence of thioglycolic acid-stabilized CdZnSeS/ZnS quantum dots (QDs). Luminescence quenching was studied in model solutions and human blood plasma to establish optimal determination parameters. Optimal conditions for Dox detection were selected: QDs with optical density A = 0.05 and 25-fold plasma dilution. The developed method was applied to analyze a human blood sample. The limit of detection for Dox was 0.02 µg/mL, the limit of quantification 0.18 µg/mL, the linear range 0.27–4.07 µg/mL (R > 0.96); the method showed good reproducibility (s from 1.08 to 1.19%). The method's accuracy was confirmed by HPLC with UV detection.