Modification of 3D Printed Denture Base Material by Hyaluronic Acid to Improve Healing

Objectives: To evaluate the effect of topical release of different concentrations of hyaluronic acid from 3D printed denture bases on the healing of skin wounds. Materials and Methods: Fifteen healthy adult male rabbits from New Zealand were randomly split into three groups based on how long they would be kept: 3 days, 7 days, and 14 days. We shaved the skin on the back of each rabbit and then made five cuts in it. The wounds were sutured to allow for healing. The animals were split into three groups based on their treatment type. The first group had their wounds treated with just an incision (control negative), the second group had their incisions treated with a 3D printed specimen under the skin incision without hyaluronic acid (control positive). We added 0.25% hyaluronic acid and a 3D printed denture base specimen to the third incision, and we added 0.5% hyaluronic acid and a 3D printed denture base to the fourth incision. The fifth cut was treated with 1% HA acid and a 3D printed denture base specimen. After that, the biopsies were sent for immunohistochemistry evaluation. Results: The data from the treated group showed that hyaluronic acid (HA) speeds up healing. Conclusion: HA-treated groups, particularly those administered 0.5% and 1% concentrations, demonstrated markedly accelerated and morecomprehensive epithelial regeneration.