SIRT-1 activation by quercetin opposes the actions of three transcription factors: p53, ATF4, and NF-κB in a renal ischaemia reperfusion injury in rats

The results showed that Q preserved the kidney functions from via acting as antioxidant by upregulating the superoxide dismutase and SLC7A11 levels, downregulating the inflammatory markers, NF-κB, TNF-α, as well as suppressing ATF4/CHOP, and p53/miR34-a/p66Shc/caspase 3 apoptotic pathways. However, the use of EX527 showed a surge of the inflammatory and apoptotic responses and a depletion of renal antioxidant capacity; thus, reversing the observed protective actions to suggest the significant role of SIRT-1 activation by Q, still potential off-targets actions of the former cannot be excluded.