A web-based atlas for exploring post-transcriptional regulation in the archaeon <i>Sulfolobus acidocaldarius</i>

Post‑transcriptional regulation is a key control layer in gene expression. Yet, resources integrating antisense RNAs (asRNAs), RNA processing sites, and RNA-protein interactions are scarce for archaeal organisms. Here, we combine multiple RNA‑seq strategies and RIP‑seq to expand the <i>Sulfolobus acidocaldarius</i> transcriptome with 1,048 asRNAs, thousands of transcript processing sites, and the interactomes of the essential RNA chaperones Sm-like archaeal protein (SmAP)1 and SmAP2. Integrating the novel generated data for the re‑analysis of heat‑shock transcriptomics reveals a consistent upregulation of asRNAs and antagonistic expression profiles with their cognate mRNAs. Moreover, our publicly accessible web atlas provides a community platform to explore these datasets and assist in the formulation of new hypotheses about archaeal RNA regulation.