Multiplex CRISPR/Cas9-mediated editing of seven glycosyltransferase homologs in Nicotiana benthamiana to produce stable, Cas9-free, glycoengineered plants
This work establishes the first <i>N. benthamiana</i> lines that are fully Cas9-free and homozygously edited at all seven key glycosyltransferase loci. These glycoengineered lines provide a stable and versatile genetic platform for future plant-based glycoengineering efforts and the production of recombinant therapeutic proteins.
Authors
- Hyangju Kang (ORCID: https://orcid.org/0000-0003-3417-3762)
- Ok Jae Koo (ORCID: https://orcid.org/0000-0001-6399-6472)
- Eun‐Ju Sohn (ORCID: https://orcid.org/0000-0002-4305-9462)
- Geung‐Joo Lee (ORCID: https://orcid.org/0000-0002-3774-1860)
- Yidong Ran (ORCID: https://orcid.org/0000-0002-4201-3964)
- Chetan Kaur (ORCID: https://orcid.org/0000-0001-6226-7921)
- Hayoung Song
- Seoyoung C. Kim (ORCID: https://orcid.org/0000-0002-2517-3579)
- Myungjin Lee (ORCID: https://orcid.org/0000-0002-9183-8559)
- Dong-Hoon Seo
Institutions
- Chungnam National University (KR)
- Kyungpook National University (KR)
- Biodesigns (United States) (US)
- Pohang TechnoPark (South Korea) (KR)
Publication Details
- Journal
- Frontiers in Plant Science
- Published
- 2025-12-12
- DOI
- https://doi.org/10.3389/fpls.2025.1701668
- Citations
- 1
- Primary Topic
- Transgenic Plants and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.23
Funders
- Rural Development Administration