Measuring the Oscillation Frequency beyond the Diffraction Limit

High-resolution array detectors are widely used in single-particle tracking, but their performance is limited by excess noise from background light and dark current. As pixel resolution increases, the diminished signal per pixel exacerbates susceptibility to noise, degrading tracking accuracy. To overcome this limitation, we use spatial-mode demultiplexing (SPADE) as a noise-robust approach for estimating the motion characteristics of an optical pointlike source. We show that SPADE efficiently concentrate the information into a few key spatial modes, drastically reducing the number of detectors while maintaining high estimation precision. Furthermore, we enhance the robustness of the estimation against excess noise by elaborately designing the modes to be decomposed. We demonstrate, both theoretically and experimentally, that a SPADE with two specific modes outperforms direct imaging in estimating the microoscillation frequency of an optical point source in the presence of excess noise.

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Publication Details

Journal
Physical Review Letters
Published
2025-11-21
DOI
https://doi.org/10.1103/szcc-vk9r
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
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article

Measuring the Oscillation Frequency beyond the Diffraction Limit

Xiao-Ming Lu, Jun Xin, Chang-Yi Hu
Physical Review Letters
Advanced Fluorescence Microscopy Techniques
article

Measuring the Oscillation Frequency beyond the Diffraction Limit

Xiao-Ming Lu, Jun Xin, Chang-Yi Hu
article en

Abstract

High-resolution array detectors are widely used in single-particle tracking, but their performance is limited by excess noise from background light and dark current. As pixel resolution increases, the diminished signal per pixel exacerbates susceptibility to noise, degrading tracking accuracy. To overcome this limitation, we use spatial-mode demultiplexing (SPADE) as a noise-robust approach for estimating the motion characteristics of an optical pointlike source. We show that SPADE efficiently concentrate the information into a few key spatial modes, drastically reducing the number of detectors while maintaining high estimation precision. Furthermore, we enhance the robustness of the estimation against excess noise by elaborately designing the modes to be decomposed. We demonstrate, both theoretically and experimentally, that a SPADE with two specific modes outperforms direct imaging in estimating the microoscillation frequency of an optical point source in the presence of excess noise.

Physical Review LettersVol. 135(24)
Hangzhou Dianzi University (CN)
Openalex Percentile: Top 73%
Advanced Fluorescence Microscopy Techniques
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