Hot-Air Drying Temperature Affects Physiological Performance and Cyto(geno)toxic Endpoints in Soybean Seeds

Soybeans are widely used in agro-industrial sectors, and global demand for this crop continues to rise. After harvest, however, soybean seeds often lack the appropriate moisture content for storage, making drying a common practice under changing climate conditions. Because temperature is a critical factor during drying, this study aimed to evaluate the effect of air-drying temperature on physiological responses and cytogenetic conformation of soybean seeds. The experiment was conducted under a completely randomized design with four replications for each temperature. Seeds with 23 percent moisture content were dried in a convective dryer equipped with airflow and temperature control at 40 °C, 50 °C, 60 °C, and 70 °C until reaching 13 percent. Samples for physiological and cytological analyses were collected before and after drying. The results indicated that drying temperature influenced seed performance and vigor. Moreover, nuclear alterations were identified as an important component of the genotoxicity caused by high drying temperatures. Overall, air temperatures above 50 °C induced physiological and cytogenotoxic effects, underscoring the need for careful monitoring during seed drying.