Construction of the indicator-free electrochemical biosensor with magnetically self-assembly based on Fe3O4/α-Fe2O3 magnetic heterogeneous nanorods for the ultra-sensitive detection of CYFRA 21-1 DNA
The results showed that the detection range of CYFRA 21-1 DNA was 10 pM-10 μM, the limit of detection (LOD) was 1.5 pM. The biosensor exhibited excellent selectivity, reproducibility, and stability; the relative standard deviation (RSD) was 2.01%. The average recovery rate in the spiked diluted human serum samples was 101.4%, and the RSD was ≤5.2%, indicating that the biosensor possessed promising prospect.
Authors
- Zhanao Wu
- Jiawei Wang (ORCID: https://orcid.org/0000-0002-1847-7267)
- Zhou Wang (ORCID: https://orcid.org/0000-0002-3616-4029)
- Hongfei Wang (ORCID: https://orcid.org/0000-0002-7828-9428)
- Peng Deng (ORCID: https://orcid.org/0000-0001-5781-090X)
- X.Y. Yi
Institutions
- Jiangsu University (CN)
- Nantong University (CN)
- Affiliated Hospital of Nantong University (CN)
- Panzhihua University (CN)
- Affiliated Hospital of Jiangsu University (CN)
- Zoucheng People's Hospital (CN)
- Zhenjiang City Fourth People's Hospital (CN)
Publication Details
- Journal
- Frontiers in Chemistry
- Published
- 2025-12-12
- DOI
- https://doi.org/10.3389/fchem.2025.1696542
- Citations
- 1
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.81