Combinatorial genome engineering of pseudorabies virus Bartha by developing a reverse genetic system based on three overlapping genomic segments
The reverse genetic system developed in this study enables rapid and combinatorial engineering of viruses with the large DNA genome, and will accelerate development of large DNA virus-based therapeutics including live-attenuated vaccines, vector vaccines, and oncolytic herpesviruses.
Authors
- Hailong Wang (ORCID: https://orcid.org/0000-0001-9874-5892)
- Yue Yang (ORCID: https://orcid.org/0000-0002-1481-8175)
- Ji Luan (ORCID: https://orcid.org/0000-0001-9553-247X)
- Hengxing Yuan
- Yulu Zhang (ORCID: https://orcid.org/0000-0001-6993-9049)
- Tan Long
Institutions
- Shandong University (CN)
- Helmholtz Centre for Infection Research (DE)
Publication Details
- Journal
- Journal of Advanced Research
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1016/j.jare.2025.12.013
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00