Structural revision of burkholdine-1215, a cyclic octapeptide isolated from <i>Burkholderia</i>

We revised a structure of burkholdine (Bk)-1215, a cyclic octalipopeptide isolated from <i>Burkholderia</i>. We synthesised both α- and β-isomers of 1-methylethyl-D-xylopyranoside and compared them to the isolated moiety by NMR analysis. SPARTAN simulation was then performed to establish the stereochemistry of the anomeric position as the β-isomer. Additionally, the NMR assignment of the ATHOD moiety was revised using 2D-NMR analysis. Finally, the γ-linkage between the D-Dab and β-OHTyr residues was determined.