EVALUATING THE ANTIBIOTIC POTENTIAL OF METABOLIC PEPTIDES DERIVED FROM LACTOBACILLUS PLANTARUM USING IN SILICO APPROACH

The increasing resistance of microorganisms to conventional antibiotics resulted in rising interest in the use of probiotic microorganisms and their antimicrobial metabolites as alternatives to antibiotics. Lactobacillus species are generally considered to be safe probiotic microorganisms. This study evaluated the antibiotic potential of metabolic peptides derived from Lactobacillus plantarum using computational methods. Genomic data on Lactobacillus plantarum with accession no. GCA_001434175.1 was retrieved from sequence raw archive of National Center for Biotechnology Information for analysis. Genome assembly was done using the database of Bacterial Viral Bioinformatics Resource Centre. Metabolic peptides were identified using antiSMASH server. Antimicrobial regions of the metabolic peptides were predicted using the database of Collection of Antimicrobial Peptides (CAMPR3). The antimicrobial regions were evaluated for antibiotic activity using the Antibiotic prediction server. The identified metabolic peptides were T3PK3, terpene precursor, terpene, RiPP-like and cyclic lactone- auto inducer. Various regions of the metabolic peptides indicated antimicrobial probabilities of 0.87, 0.78, 0.89, 0.95 and 0.97 respectively. Findings of this study demonstrated antimicrobial potential of metabolic peptides derived from Lactobacillus plantarum.