Molecular and Biological Detection of Streptococcus mutans Bacteria for Iraqi patients

The present work aimed to isolate and identifying S. mutans that isolated from saliva by using PCR technique. Saliva was collected from forty healthy subjects in Baghdad city; ages were between 19-21 years old. Before beginning the research, approval of the protocol was acquired from the relevant Ethical Committee, and each subject gave their written permission after receiving appropriate information. The results showed 100 healthy patients of Dentistry College from Baghdad University were included in this study, 65% of them were male and 35% were female, 87% were residents of Baghdad and 13% were non-Baghdad residents. In order to verify the identity of the bacteria, an amplification of the 16S rRNA was carried out using 12 separate samples. After using primers targeting a conserved area of 16S rRNA to amplify the DNA of S. mutans isolates using PCR.