Context‐Specific Metabolic Alterations in HPRT1 Knockout Cells Within a 3D Culture System
This study highlights the potential of 3D culture systems to elucidate context-specific mechanisms of metabolic regulation, such as the suppressive effect of HPRT1 on carnosine production. Our findings demonstrate that metabolic phenotypes observed in 2D cultures may not fully capture the complexity of in vivo metabolism, whereas 3D models can reveal regulatory pathways that are otherwise overlooked, including context-dependent regulation of carnosine metabolism by HPRT1.
Authors
- Sho Tabata (ORCID: https://orcid.org/0000-0001-8956-9241)
- Tomoyoshi Soga (ORCID: https://orcid.org/0000-0001-9502-2509)
- Ichiro Fujimoto (ORCID: https://orcid.org/0000-0001-7088-6407)
- Hideki Makinoshima (ORCID: https://orcid.org/0009-0001-8181-6241)
Institutions
- Kaken Pharmaceutical (Japan) (JP)
- National Cancer Center Hospital East (JP)
- National Institute of Technology, Tsuruoka College (JP)
Publication Details
- Journal
- Cancer Medicine
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1002/cam4.71452
- Primary Topic
- Biochemical effects in animals
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Society for the Promotion of Science