Cold argon plasmas for non-enzymatic digestion of macromolecules
We have developed surface treatment techniques utilising radiofrequency current cold argon plasmas (RF-CAP) for non-enzymatic digestion of macromolecules, exemplified here with peptides and proteins of varying sizes. These plasmas are able to rapidly digest condensed-phase macromolecules with minimal sample preparation, producing predictable fragments visible by LC-MS analysis.
Authors
- Dániel Simon (ORCID: https://orcid.org/0000-0001-5056-913X)
- Felicia M. Green (ORCID: https://orcid.org/0000-0002-9444-2701)
- Daniel McGill (ORCID: https://orcid.org/0000-0002-3100-8377)
- Sivaramakrishnan Ramadurai (ORCID: https://orcid.org/0009-0006-3664-1573)
- Zoltán Takáts (ORCID: https://orcid.org/0000-0002-0795-3467)
Institutions
- Rutherford Appleton Laboratory (GB)
- Hammersmith Hospital (GB)
- Rosalind Franklin Institute (GB)
- Imperial College London (GB)
- University of Regensburg (DE)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-12-02
- DOI
- https://doi.org/10.1039/d5cc06333g
- Primary Topic
- Plasma Applications and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- UK Research and Innovation
- Engineering and Physical Sciences Research Council