Development and Evaluation of an Optimised Sanger-Based Assay for HIV-1 Drug Resistance Genotyping in Chinese Circulating Strains Across Subtypes and Viral Loads
The optimized system improves amplification sensitivity and mutation coverage, especially in low-VL samples. It enables stable detection of key NNRTI resistance mutations and shows strong subtype compatibility, supporting its utility in clinical resistance surveillance and early detection.
Authors
- Haibing Xiao (ORCID: https://orcid.org/0000-0001-8181-0125)
- Fujie Zhang (ORCID: https://orcid.org/0000-0001-6386-9879)
- Hanxi Zhang (ORCID: https://orcid.org/0000-0002-2193-9581)
- Di Mao (ORCID: https://orcid.org/0000-0002-7950-7669)
- Mengying Li (ORCID: https://orcid.org/0009-0007-7105-7425)
- Fengting Yu
- Xi Chen (ORCID: https://orcid.org/0000-0002-8129-755X)
- Fei Liu (ORCID: https://orcid.org/0000-0003-1175-4070)
Institutions
- Capital Medical University (CN)
- Sinovac Biotech (CN)
- Beijing Ditan Hospital (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Infection and Drug Resistance
- Published
- 2025-12-01
- DOI
- https://doi.org/10.2147/idr.s506567
- Primary Topic
- HIV/AIDS drug development and treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Capital Medical University
- Beijing Municipal Administration of Hospitals