Method development and validation for simultaneous analysis of four bile acids as CYP3A drug–drug interaction biomarkers
This is the first report describing the development and validation of a 4-in-1 LC - MS/MS method for the simultaneous quantification of DCA, 1β-OH-DCA, 1β-OH-G-DCA, and 1β-OH-T-DCA in human plasma. The assay provides a robust bioanalytical platform for evaluating CYP3A-mediated DDIs and offers a methodological framework applicable to other multiplexed methods for endogenous biomarkers.
Authors
- Ryan McGee (ORCID: https://orcid.org/0009-0003-7902-0134)
- David L. Rodrigues (ORCID: https://orcid.org/0000-0001-5921-7819)
- Zhiyin Xun (ORCID: https://orcid.org/0000-0002-9793-4429)
- Phillip Wang (ORCID: https://orcid.org/0009-0007-0400-514X)
- Lin Zhang (ORCID: https://orcid.org/0009-0006-0896-8392)
- Bob Stratford (ORCID: https://orcid.org/0000-0002-9735-0220)
Institutions
- Incyte (United States) (US)
Publication Details
- Journal
- Bioanalysis
- Published
- 2025-12-02
- DOI
- https://doi.org/10.1080/17576180.2025.2601040
- Primary Topic
- Drug Transport and Resistance Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00