A simplified low-cost and reliable plant genomic DNA extraction method for PCR-based genotyping and screening
This streamlined protocol provides a cost-effective, reliable, and scalable approach for extracting plant genomic DNA suitable for PCR-based genotyping, marker development, and diversity analysis. Its simplicity and throughput make it particularly valuable for breeding programmes, although it is not intended for applications requiring highly pure DNA, such as whole-genome resequencing.
Authors
- Duncan Scholefield
- Julie King (ORCID: https://orcid.org/0000-0002-7699-7199)
- Stephen Ashling
- Caiyun Yang (ORCID: https://orcid.org/0000-0002-0521-4230)
- Surbhi Grewal (ORCID: https://orcid.org/0000-0002-0707-2504)
- Ian P. King
Institutions
- University of Nottingham (GB)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-12-10
- DOI
- https://doi.org/10.1186/s13007-025-01480-8
- Citations
- 1
- Primary Topic
- Genetic diversity and population structure
- Type
- article
- Field-Weighted Citation Impact
- 1.82