An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice
Swine acute diarrhea syndrome coronavirus (SADS-CoV), an emerging bat-origin Alphacoronavirus with demonstrated zoonotic potential, poses a significant threat to swine health and has considerable economic implications. Currently, no licensed vaccines are available. We constructed a replication-deficient human adenovirus type 5 (Ad5) vectored vaccine candidate, rAd5-SADS-S, which expresses the SADS-CoV spike (S) glycoprotein. The rAd5-SADS-S vaccine elicited robust SADS-CoV-specific humoral immunity and potent cellular responses in both mice and pigs. Notably, rAd5-SADS-S conferred passive protection to neonatal mice against lethal SADS-CoV challenge. These findings establish a preclinical foundation for the development of SADS-CoV vaccines.
Authors
- Cong Yuan (ORCID: https://orcid.org/0000-0002-1003-5819)
- Yu Zhang (ORCID: https://orcid.org/0000-0001-5887-9239)
- Juan Zhang (ORCID: https://orcid.org/0000-0002-5050-9601)
- Yueyue Duan
- Xueliang Zhu (ORCID: https://orcid.org/0000-0001-8019-9336)
- Qi Wang (ORCID: https://orcid.org/0000-0001-6942-2410)
- Haixue Zheng (ORCID: https://orcid.org/0000-0001-6850-1379)
- Lei Shi (ORCID: https://orcid.org/0000-0002-6043-8819)
- Luyao Li (ORCID: https://orcid.org/0000-0003-1397-5045)
- Wei Mao (ORCID: https://orcid.org/0000-0003-3804-2380)
- Maowen Sun (ORCID: https://orcid.org/0009-0007-1677-7155)
Institutions
- Lanzhou Institute of Chemical Physics (CN)
- Lanzhou Veterinary Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Sichuan Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Virologica Sinica
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1016/j.virs.2025.12.007
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00